Cell line/type | CRL-1439 (epithelial cell line) |
---|---|
Species | Rat |
Animal free | Yes |
Product | Completeā¢ |
Latinwo, L. M., Badisa, V. L., Odewumi, C. O., Ikediobi, C. O., Badisa, R. B., Brooks-Walter, A., ... & Nwoga, J. (2008). Comparative evaluation of cytotoxicity of cadmium in rat liver cells cultured in serum-containing medium and commercially available serum-free medium. International journal of molecular medicine, 22(1), 89-94. In this report, the toxic effect of cadmium (0-300 microM) on cell growth, total RNA, total proteins, and antioxidant enzymes were compared in rat normal liver cells cultured in medium with 10% FBS or commercially available serum-free medium for 4 or 8 hours. With cadmium concentration at above 100 μM, the total levels of RNA, protein and cell growth decreased in serum-containing medium, while their levels increased in serum-free medium compared to the controls. The glutathione peroxidase and glutathione reductase levels were lower in serum-free medium than in serum-containing medium, indicating less oxidative stress in cells grown in serum-free medium. These results clearly suggest that cadmium showed higher toxicity to liver cells grown in serum-containing medium in comparison to commercially available serum-free medium. Complete™ is a commercially available serum-free medium The precise formulation is proprietary. https://www.spandidos-publications.com/ijmm/22/1/89/download |
|
Source | Literature - commercial |
Chemically defined > Unspecified | Unspecified |