Cell line/type | DRG (dorsal root ganglia) neurons |
---|---|
Species | Rat |
Animal free | Yes |
Product | Neurobasal Lui et al. 2008 |
Liu, J., Rumsey, J. W., Das, M., Molnar, P., Gregory, C., Riedel, L., & Hickman, J. J. (2008). Electrophysiological and immunocytochemical characterization of DRG neurons on an organosilane surface in serum-free medium. In Vitro Cellular & Developmental Biology-Animal, 44(5-6), 162-168. In this study, the aim was to recreate a stretch reflex circuit on a patterned Bio-MEMS (bio-microelectromechanical systems) chip with deflecting micro-cantilevers. To create the in vitro stretch reflex arc, the first step is to culture the components of the reflex arc system in a defined environment. In this paper, the authors have evaluated the growth of sensory neurons on this synthetic silane substrate. The culture medium was neurobasal supplemented with different components as specified in the Materials and Methods, under the heading; Rat embryonic DRG neuron culture. The formulation of Neurobasal can be found online: https://www.thermofisher.com/nl/en/home/technical-resources/media-formulation.251.html. https://link.springer.com/content/pdf/10.1007%2Fs11626-008-9097-x.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |