Cell line/type | CHO (Chinese Hamster Ovary) cell line |
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Species | Hamster |
Animal free | Unspecified |
Product | ProCHO4 Astley et al. 2007 |
Astley, K., Naciri, M., Racher, A., & Al-Rubeai, M. (2007). The role of p21cip1 in adaptation of CHO cells to suspension and protein-free culture. Journal of biotechnology, 130(3), 282-290. In this study, to identify the potential role of p21 in adaptation to suspension and protein-free cultures, the survival and growth of anchorage- and serum-dependent CHO cell lines was studied that differed only in the period of p21-induced arrest. p21 overexpression led to rapid adaptation of cells to suspension and protein-free cultures. In contrast, following adaptation to protein-free growth media, an overall increase in specific productivity was observed. The ability of cells to survive in protein-free suspension cultures was due to the requirement of G1 cells to growth factors and to their relatively high resistance to the hydrodynamic forces. The cells were cultured in a protein-free medium, ProCHO4. ProCHO4 is a commercial product The precise formulation is proprietary. https://ac.els-cdn.com/S0168165607003227/1-s2.0-S0168165607003227-main.pdf?_tid=3a3a4eaa-34f1-466d-b5a2-8e6d2ce3408e&acdnat=1534430185_67203701d0182f7637e6576d0ac0a008 |
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Source | Literature - modified commercial product |
Contains phenol red > No | No |
Antibiotics free > Unspecified | Unspecified |