Cell line/type | CHO (Chinese Hamster Ovary) cell line |
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Species | Hamster |
Animal free | Yes |
Product | DMEM/F12 Liu et al. 2009 |
Liu, C. H., & Wu, K. W. (2009). Synergistic effects of basic fibroblast growth factor and insulin on Chinese hamster ovary cells under serum-free conditions. Journal of bioscience and bioengineering, 107(3), 312-317. The aim of this study was to find new growth factors that could maintain viability and enhance the proliferation of CHO cells under serum-free conditions. Macrophage colony-stimulating factor (M-CSF)-secreting CHO cells were used as a model cell line to evaluate the stimulatory effects of different compounds, including two growth factors, on cell proliferation. Simultaneous supplementation with insulin and bFGF synergistically promoted the growth of CHO cells and recombinant M-CSF synthesis in basal DMEM/F12 medium. The mitogenic effects of bFGF and insulin under serum-free conditions were confirmed in the CHO-K1 cell line. These results provide useful information for the design and development of serum-free media as well as for antiapoptotic studies of CHO cells under serum-free conditions. In conclusion, insulin and bFGF synergistically promoted the proliferation of CHO cells and recombinant M-CSF synthesis in basal DMEM/F12 medium. The protocol and final formulation of the serum-free, DMEM/F12 based medium are listed in the Materials and Methods and the Results. https://ac.els-cdn.com/S138917230900070X/1-s2.0-S138917230900070X-main.pdf?_tid=730f6087-d8d4-47d1-8a99-12282e307893&acdnat=1534239958_df370e29cf95ca2946d8bf308dfe0af2 |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |