Cell line/type | CHO (Chinese Hamster Ovary) cell line |
---|---|
Species | Hamster |
Animal free | Unspecified |
Product | CHO-S-SFM II Kutsuzawa et al. 2013 |
Kutsuzawa, K., Takahashi, C., Sato, R., Suzuki, T., Kishimoto, H., Murakami, A., ... & Otsuka, H. (2013). Multiarray formation of CHO spheroids cocultured with feeder cells for highly efficient protein production in serum-free medium. Journal of nanoscience and nanotechnology, 13(1), 229-235. This study reports on a new three-dimensional cell culture system as a spheroid cell culture on the micropattern array for efficient production of protein in CHO cells. Furthermore, cocultivation of CHO spheroids with feeder cells including bovine aortic endothelial cells (BAEC) and NIH 3T3 was essential to more increase a protein production. The results indicated that CHO heterospheroids cocultured with BAECs were much superior to either CHO monolayers or CHO homospheroids in protein production. Significantly, the above cocultured spheroids in the serum-free medium drastically enhanced protein expression level up to 3-fold compared with CHO spheroids in serum medium, suggesting that a coculture of spheroid system with feeder layer cells is a promising method to enhance protein production under serum-free condition. The cells were cultured in CHO-S-SFM II. CHO-S-SFM II is a commercially available product. The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pubmed/23646721 |
|
Source | Literature - commercial |
Chemically defined > Unspecified | Unspecified |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |