Cell line/type | CHO (Chinese Hamster Ovary) cell line |
---|---|
Species | Hamster |
Animal free | Unspecified |
Product | SAFC serum free media |
From: Elhofy, A. (2016). Novel Cell-Ess® supplement used as a feed or as an initial boost to CHO serum free media results in a significant increase in protein yield and production. Computational and structural biotechnology journal, 14, 319-324. In this study, the authors investigated if the addition of a lipid supplement, Cell-Ess®, would lead to an increase in protein production across different clones in two different genetic backgrounds of CHO cell lines. Cells were either grown in SAFC serum-free media or FortiCHO. At a concentration of 1% (v/v) the lipid supplement caused a significant increase in protein titer (1118 ± 65.4 ng 105 cells−1 days−1 ) when compared to cultures grown in the absence of supplementation (819.3 ± 38.1 ng 105 cells−1 days−1 ; p b 0.05). This equated to a 37% increase in productivity. Furthermore, metabolic profiles of ammonia, glutamate, lactate, and glucose were not significantly altered by the polar lipid supplement. SAFC serum-free media or FortiCHO are proprietary, commercial available products. http://www.sciencedirect.com/science/article/pii/S200103701630023X |
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Source | Literature - modified commercial product |
Chemically defined > Unspecified | Unspecified |
Elhofy (2016) - Elhofy, A. (2016). Novel Cell-Ess® supplement used as a feed or as an initial boost to CHO serum free media results in a significant increase in protein yield and production. Computational and structural biotechnology journal, 14, 319-324. |