Cell line/type | CHO (Chinese Hamster Ovary) cell line |
---|---|
Species | Hamster |
Animal free | Yes |
Product | CD DG44-hydrolysates |
Azarian, B., Sajedin, S. M., Azimi, A., Raigani, M., Vaziri, B., & Davami, F. (2017). Proteomics Profiling of Chimeric-Truncated Tissue Plasminogen activator Producing-Chinese Hamster Ovary Cells Cultivated in a Chemically Defined Medium Supplemented with Protein Hydrolysates. Iranian biomedical journal, 21(3), 154. In this study, designing the optimum mixture of four soy and casein-derived hydrolysates was successfully performed by design of experiment and specific productivity increased in all predicted combinations. Protein profile of recombinant CHO (rCHO) cells producing tissue plasminogen activator in a serum-free medium (SFM) supplemented with designed hydrolysate additives was compared to that of rCHO cells cultivated in SFM. Identification of differentially expressed proteins using two-dimensional gel electrophoresis coupled with MALDI-TOF/TOF revealed the role of energy metabolism related proteins and importance of prevention of oxidative stress by this special media enrichment strategy. Up-regulation of mitochondrial enzymes, pyruvate dehydrogenase E1, and Peroxiredoxin-III, as well as other proteins involved in metabolic pathways, and uridine monophosphate/cytidine monophosphate kinase indicated higher metabolic activity. Furthermore, along with antioxidant effect of peptones, proteins with antioxidant function such as ferritin and peroxiredoxin-III were up-regulated. SFM was supplemented with the hydrolysates with the final concentrations of 2 and 5 g L−1. CD DG44 medium is a commercial product. The precise formulation is proprietary https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5392218/pdf/IBJ-21-154.pdf |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |