Cell line/type | BMM (bone marrow derived macrophages) |
---|---|
Species | Murine (Mouse or rat) |
Animal free | No |
Product | RPMI 1640-Panexin BMM® |
Eske, K., Breitbach, K., Köhler, J., Wongprompitak, P., & Steinmetz, I. (2009). Generation of murine bone marrow derived macrophages in a standardised serum-free cell culture system. Journal of immunological methods, 342(1-2), 13-19. The aim of this study was to establish a cell culture system for the generation of murine BMM (bone marrow derived macrophages) under standardised serum-free conditions. The use of a newly developed compositionally defined serum supplement enabled us to gain mature BMM from BALB/c and C57BL/6 mice expressing the myeloid marker F4/80, CD11b and MOMA-2. Under these serum-free conditions, LPS and IFN-gamma stimulated C57BL/6 BMM released more IL-12 and nitric oxide (NO) compared to BALB/c BMM whereas the latter cells produced higher levels of IL-10 and MCP-1 after LPS stimulation. Serum-free generated C57BL/6 BMM showed enhanced bactericidal activity against the Gram-negative rod Burkholderia pseudomallei compared to BALB/c BMM. In conclusion, the serum-free generation of BMM described in this study will assure more standardised and reproducible conditions for the future characterisation of murine BMM. The serum-free medium for culturing BMM consisted of RPMI 1640 supplemented with 5% Panexin BMM®, 50 μM mercaptoethanol and 2 ng/ml recombinant murine GM-CSF without the addition of antibiotics as specified in the Materials and Methods, under the heading; 2.1. Serum-free cell culture medium. Panexin BMM® is a commercially available supplement. The precise formulation of Panexin BMM® is proprietary. https://ac.els-cdn.com/S0022175908003542/1-s2.0-S0022175908003542-main.pdf?_tid=355f0a01-4ce4-4637-ba43-5c701b3147e3&acdnat=1534241969_06e70cf2b16c226a64ccd539cd5a179b |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |