Cell line/type | EGC (embryonic germ cells) |
---|---|
Species | Human |
Animal free | No |
Product | DMEM-KSR medium Hua et al 2009 |
Hua, J., Yu, H., Liu, S., Dou, Z., Jing, X., Yang, C., ... & Gao, Z. (2009). Derivation and characterization of human embryonic germ cells: serum-free culture and differentiation potential. Reproductive biomedicine online, 19(2), 238-249. This study examined the effects of a chemically defined culture medium supplement, knock-out serum replacement (KSR), on the growth and differentiation of human embryonic germ cells (hEGC) and found that the efficiency of the initial establishment of hEGC lines in KSR medium was significantly higher than in fetal calf serum (FCS) medium. The percentage of undifferentiated hEGC colonies growing in KSR medium was significantly higher than in FCS-based medium (P < 0.05). In conclusion, an appropriate serum-free culture system has been developed for the establishment of hEGC lines. DMEM medium was supplemented with KSR and other components as specified in the Materials and Methods, under the heading; Primary cultures of human and mouse primordial germ cells. https://ac.els-cdn.com/S147264831060079X/1-s2.0-S147264831060079X-main.pdf?_tid=ef3dfa1f-a435-4173-b117-d4efd8328a88&acdnat=1534241963_7e5a9c3a00ce19ef16d60201fce67625 |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |