Cell line/type | EGC (embryonic germ cells) |
---|---|
Species | Porcine (Pig) |
Animal free | No |
Product | DMEM-KSR Petkov et al. 2008 |
Petkov, S. G., & Anderson, G. B. (2008). Culture of porcine embryonic germ cells in serum-supplemented and serum-free conditions: the effects of serum and growth factors on primary and long-term culture. Cloning and stem cells, 10(2), 263-276. The purpose of this study was to determine if FBS can be replaced with Knockout Serum Replacement (KSR), a defined medium supplement, and to examine the effects of FBS and growth factors on short- and long-term culture of pig embryonic germ cells (EGC). No significant differences were observed in total and mean colony areas in primary cultures between FBS- and KSR-supplemented medium. Total and mean colony areas were significantly larger in KSR-supplemented medium compared with medium supplemented with KSR and growth factors. The cultures proliferated for significantly higher numbers of passages in FBS-supplemented medium and in medium supplemented with KSR and growth factors compared with medium containing KSR alone. Porcine EGC maintained in serum-free conditions were positive for pluripotent stem cell markers, maintained stable karyotypes for up to 54 passages, and were capable of differentiating in vitro into cells from the three primary germ layers. In conclusion, KSR was successfully employed as a medium supplement to establish porcine EGC lines with high efficiency. The embryonic germ cells were cultured in DMEM supplemented with KSR as specified in the Materials and Methods, under the heading; Isolation and culture of PGC. https://www.liebertpub.com/doi/pdfplus/10.1089/clo.2007.0085 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |