Cell line/type | Hybridoma 84 |
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Species | Hybridoma |
Animal free | Yes |
Product | BTI-DMEM/F12 medium |
Lee, J., Tscheliessnig, A., Chen, A., Lee, Y. Y., Adduci, G., Choo, A., & Jungbauer, A. (2009). Adaptation of hybridomas to protein-free media results in a simplified two-step immunoglobulin M purification process. Journal of Chromatography A, 1216(13), 2683-2688. In this study, an IgM antibody was purified from hybridoma supernatant containing serum using a three-step purification process comprising of tangential flow filtration, anion-exchange chromatography and size-exclusion chromatography. Recovery and purity were significantly improved upon adaptation of the hybridoma to serum-free media. The process could even be simplified by omitting the initial tangential flow filtration step. Even with a two-step purification process a purity of >98% and a recovery of >60% was obtained. The formulation of the serum-free, DMEM/F12 based medium is BTI proprietary. https://ac.els-cdn.com/S0021967308018438/1-s2.0-S0021967308018438-main.pdf?_tid=8e6bdcd8-db30-46a2-adc1-5ff249da54e0&acdnat=1534241953_dd849be2212202eaf443e7a99fa7cda6 |
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Source | Literature - commercial |
Chemically defined > Yes | Yes |