Cell line/type | Foetal femur-derived cells |
---|---|
Species | Human |
Animal free | No |
Product | IMDM/F12 Mirmalek‐Sani et al. 2009 |
Mirmalek‐Sani, S. H., Stokes, P. J., Tare, R. S., Ralph, E. J., Inglis, S., Hanley, N. A., ... & Oreffo, R. O. (2009). Derivation of a novel undifferentiated human foetal phenotype in serum‐free cultures with BMP‐2. Journal of cellular and molecular medicine, 13(9b), 3541-3555. In this study, human foetal femur-derived cells maintained in a chemically defined medium (CDM) with activin A and fibroblast growth factor-2 generated a unique undifferentiated cell population in comparison to basal cultures, with significantly reduced amino acid depletion, appearance and turnover, reduced alkaline phosphatase (ALP) activity and loss of type I and II collagen expression demonstrated by fluorescence immunocytochemistry. These studies demonstrate the use of an intermediary foetal cellular model for differentiation studies in chemically defined conditions and indicate the in vitro reconstruction of the mesenchymal condensation phenotype in the presence of BMP-2, with implications therein for rescue studies, screening assays and skeletal regeneration research. The formulation of the chemically defined, serum-free, IMDM/F12 based medium is listed in the Materials and Methods, under the heading; Chemically defined medium. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430854/pdf/jcmm0013-3541.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |