Cell line/type | Pancreatic islets |
---|---|
Species | Rat |
Animal free | Yes |
Product | RPMI1640-sericin medium |
Morikawa, M., Kimura, T., Murakami, M., Katayama, K., Terada, S., & Yamaguchi, A. (2009). Rat islet culture in serum‐free medium containing silk protein sericin. Journal of Hepato‐Biliary‐Pancreatic Surgery, 16(2), 223-228. In this study, twenty rat islets were cultured in medium containing either sericin or FBS, or no supplement, for 14 days, during which time viable islets were counted in order to evaluate islet survival. Ninety-five percent of islets were viable after culture for 14 days in culture medium supplemented with either FBS or sericin, while no islets survived beyond 7 days in culture without supplement. No significant differences in stimulated insulin secretion were noted between two groups of islets grown on supplemented media. Histologically, islets cultured with sericin displayed a well-preserved structure and strong insulin staining before and after transplantation. Serum-free medium containing sericin appears to be useful for islet culture. The formulation of the serum-free RPMI1640-based medium supplemented with sericin is listed in the Materials and Methods, under the heading; Islet culture and survival of cultured islets. http://RPMI1640-sericin medium |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |