Cell line/type | Erytrocytes |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM/F12 Niimi et al. 2009 |
Niimi, G., Ohashi, K., IDE, T., & Pereda, J. (2009). Budding and direct division of adult human erythrocytes in serum-free cultures. Okajimas folia anatomica Japonica, 86(3), 73-77. This paper documents the budding and division process of human erythrocytes in vitro using time-lapse light microscopy of hanging-drop preparations. The erythrocytes were prepared from normal adult human peripheral blood. Red blood cells showed cytoplasmic budding, segmentation, and direct division with delayed addition of erythropoietin in serum-free Dulbecco's modified Eagle's medium/nutrient mixture F-12 Ham. These observations are thought to be useful for developing model of definitive erythropoiesis and simple expansion of human erythrocytes. The used protocol is listed in the Materials and Methods. https://www.jstage.jst.go.jp/article/ofaj/86/3/86_3_73/_pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > No | No |