Cell line/type | PBMC-derived fibroblasts |
---|---|
Species | Human |
Animal free | No |
Product | FibroLife medium Pilling et al. 2009 |
Pilling, D., Vakil, V., & Gomer, R. H. (2009). Improved serum-free culture conditions for the differentiation of human and murine fibrocytes. Journal of immunological methods, 351(1-2), 62-70. In this study, enhanced serum-free conditions are described that support the rapid differentiation of human and murine fibrocytes. The effect on fibrocyte differentiation of different anti-coagulants used when collecting blood, and for culturing cells, the effects of different commercial media formulations, the addition of a variety of supplements, cell density, conditioned medium, and glass and plastic substrates are compared. Fibrocyte differentiation was enhanced when the serum-free medium was based on either FibroLife or StemPro formulations. For optimal fibrocyte differentiation, we suggest using heparin as an anti-coagulant, and culturing cells at 2.5×105cells/ml in FibroLife basal medium with glutamine, HEPES, non-essential amino acids, sodium pyruvate, albumin, insulin, transferrin, sodium selenite, linoleic acid, and oleic acid, on tissue culture plastic. FibroLife basal medium is a commercially available product. The precise formulation is proprietary. https://ac.els-cdn.com/S0022175909002919/1-s2.0-S0022175909002919-main.pdf?_tid=7fb24a0f-282d-450c-b34f-460291b6ece0&acdnat=1534237479_71c7cfe72dfffc2b60dfc1f1babd595d |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > No | No |