Cell line/type | C57/BL6J ES cells (TB6-2) |
---|---|
Species | Mouse |
Animal free | No |
Product | Modified DMEM Sato et al. 2009 |
Sato, H., Amagai, K., Shimizukawa, R., & Tamai, Y. (2009). Stable generation of serum‐and feeder‐free embryonic stem cell‐derived mice with full germline‐competency by using a GSK3 specific inhibitor. genesis, 47(6), 414-422. In this study, a new serum- and feeder-free B6 ES cells were established with full developmental potential by using leukemia inhibitory factor (LIF) and 6-bromoindirubin-3'-oxime (BIO), a glycogen synthase kinase-3 (GSK3) inhibitor. BIO treatment significantly increased the expression levels of 364 genes including pluripotency markers such as Nanog and Klf family. Unexpectedly, by aggregating or microinjecting those ES cells to each eight-cell-stage diploid embryo, germline-competent ES-derived mice were established. Furthermore, founder mice completely derived from female XO, heterozygous, or homozygous mutant B6 ES cells were directly available for intercross breeding and phenotypic analysis. The authors propose that serum- and feeder-free B6 ES cells stimulated with LIF plus GSK3 inhibitor are valuable for generating mouse models on B6 background. The formulation of the serum-free DMEM based medium is listed in the Materials and Methods, under the heading; ES Cell Culture. https://onlinelibrary.wiley.com/doi/pdf/10.1002/dvg.20514 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |