Cell line/type | T lymphocytes |
---|---|
Species | Human |
Animal free | Unspecified |
Product | Hybris-2 |
Tabakov, V. U., Litvina, M. M., Schepkina, J. V., Jarilin, A. A., & Chestkov, V. V. (2009). Studying the proliferation of human peripheral blood T lymphocytes in serum-free medium. Bulletin of experimental biology and medicine, 147(1), 120-124. In this study, the cultivation of human peripheral blood lymphocytes in serum-free medium Hybris-2 and RPMI 1640 medium with 10% fetal bovine serum in the presence of phytohemagglutinin and interleukin-2 was compared. The optimal concentration of phytohemagglutinin significantly differed in serum-free and serum-containing media (0.5 and 5 microg/ml, respectively). Both mitogens were more potent in stimulating the proliferation of lymphocytes in serum-free medium than in serum-containing medium. Strong proliferation of CD3(+) and CD4(+) T lymphocytes were observed in both media. The dynamics of other markers was similar in serum-free and serum-containing media. Hybris-2 is developped by the group of Valery V. Chestkov of the Russian Academy of Medical Sciences, Research Centre of Medical Genetics. The formulation of Hybris-2 is proprietary. https://link.springer.com/content/pdf/10.1007%2Fs10517-009-0434-1.pdf |
|
Source | Literature - commercial |
Chemically defined > Unspecified | Unspecified |