Cell line/type | C2C12 (skeletal muscle cell line) |
---|---|
Species | Mouse |
Animal free | Yes |
Product | DMEM-B27 Molnar et al. 2007 |
Molnar, P., Wang, W., Natarajan, A., Rumsey, J. W., & Hickman, J. J. (2007). Photolithographic patterning of C2C12 myotubes using vitronectin as growth substrate in serumāfree medium. Biotechnology progress, 23(1), 265-268. In our serum-free defined culture system, differentiation of C2C12 cells into myotubes required surface-bound signals such as substrate-adsorbed vitronectin or laminin. On the basis of this substrate requirement of myotube formation, we developed a photolithography-based method to pattern C2C12 myotubes, where myotubes formed exclusively on vitronectin surface patterns. In contrast to previous experiments where cell patterning was achieved by selective attachment of the cells to patterned surfaces in a medium that contained all of the factors necessary for differentiation, this study illustrates that surface patterning of a signaling molecule, which is essential for skeletal muscle differentiation in a defined system, can result in the formation of aligned myotubes on the patterns. We have shown that myotube formation by C2C12 myoblasts is highly dependent on the culture surface under serum-free The formulation of the differentiation medium (DMEM + 1% B27 supplement) is listed in the Materials and Methods, under the heading; C2C12 Culture. https://onlinelibrary.wiley.com/doi/pdf/10.1021/bp060302q |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |