Cell line/type | C2C12 (skeletal muscle cell line) |
---|---|
Species | Mouse |
Animal free | Unspecified |
Product | DMEM-DMU(I) |
Gawlitta, D., Boonen, K. J., Oomens, C. W., Baaijens, F. P., & Bouten, C. V. (2008). The influence of serum-free culture conditions on skeletal muscle differentiation in a tissue-engineered model. Tissue Engineering Part A, 14(1), 161-171. doi: 10.1089/ten.a.2007.0095. The goal of this study was to compare the influence of serum-free media on C2C12 differentiation in 3-dimensional tissue-engineered muscle constructs. Myoblast proliferation and differentiation in media containing Ultroser G (DMU), insulin-like growth factor (IGF)-I (DMI), or both (DMUI) were compared with those induced by more-traditional media containing horse serum (HS) or horse serum and IGF-I (HSI).Addition of IGF-I (HSI) to the standard DM (HS) improved myoblast differentiation in muscle constructs. Even better results were obtained using DMU and DMUI culture conditions. DMI could not induce differentiation or maintain cell viability. Serum-free culture medium supplemented with DMU or DMUI accelerates and improves myoblast differentiation in engineered muscle tissue better than the gold standard HS. The formulation of the differentiation medium supplemented with DMU or DMUI is listed in Table 1. DMUI and DMU are abbreviations for serum substitutes. DMU = 0.4% Ultroser G, DMUI = 0.4% Ultroser G + 32 ng/mL IGF-I (table 1). Ultroser G is a commercial serum substitute. The precise formulation of Ultroser G is proprietary. https://www.liebertpub.com/doi/pdfplus/10.1089/ten.a.2007.0095 |
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Source | Literature - modified commercial product |
Chemically defined > Unspecified | Unspecified |
Antibiotics free > Yes | Yes |
Contains phenol red > Yes | Yes |