Cell line/type | ASCs-derived VECs (vascular endothelial cells) |
---|---|
Species | Human |
Animal free | No |
Product | DMEM/F12 Konno et al. 2010 |
Konno, M., Hamazaki, T. S., Fukuda, S., Tokuhara, M., Uchiyama, H., Okazawa, H., ... & Asashima, M. (2010). Efficiently differentiating vascular endothelial cells from adipose tissue-derived mesenchymal stem cells in serum-free culture. Biochemical and biophysical research communications, 400(4), 461-465. In this study, a method for differentiating mouse ASCs into VECs under serum-free conditions is described. After the differentiation culture, over 80% of the cells expressed vascular endothelial-specific marker proteins and could take up low-density lipoprotein in vitro. This protocol should be helpful in clarifying the mechanisms of ASC differentiation into the VSC lineage. The authors tested 2% KSR, B27, N2, G5, or ITS; each candidate supplement was added in EBM-2 medium instead of FBS. Culture mediasuch as DMEM, IMDM, and DMEM/F12 instead of EBM-2 were examined. Finally, to determine the optimal concentration of FGF2 or VGEF, different concentrations (0, 5, 10, and 20 ng/ml) was tested for induction of endothelial cells. The optimal culture medium for the VEC induction from ASCs was determined to be DMEM/F12 medium containing 10 ng/ml FGF2, 2% ITS, and EGM-2 BulletKit (without FGF2). https://ac.els-cdn.com/S0006291X10015159/1-s2.0-S0006291X10015159-main.pdf?_tid=eff2bc90-3ef4-4a21-b5f4-c77bf6e25e00&acdnat=1534145190_9aa559468bf163c9d10fea74d9e2b1bc |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |