Cell line/type | MIA PaCa-2 cells |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM Xiao et al. 2010 |
Xiao, J., Lee, W. N. P., Zhao, Y., Cao, R., Go, V. L. W., Recker, R. R., ... & Xiao, G. G. (2010). Profiling Pancreatic Cancer–Secreted Proteome Using 15N Amino Acids and Serum-Free Media. Pancreas, 39(1), e17. In this study, a new method of determining protein turnover by labeling protein with N amino acids was used in conjunction with serum-free cell culture to profile secreted proteins that are released by MIA PaCa-2 pancreatic cancer cells in culture. The authors identified secreted proteins from cultured MIA PaCa-2 pancreatic cancer cells and their,sponse to oxythiamine (OT) treatment using 15N amino acids and serum-free media. 15N algal amino acid mixture was added to serum-free DMEM at the beginning of the experiment as in the following:Group A, cells were cultured in DMEM containing 1 mg/mL of natural amino acid as unlabeled control; group B, cells with 50% enrichment of 15N algal amino acid mixture (98% 15N); and group C, cells with 50% enrichment of 15N algal amino acid mixture (98% 15N) plus 10 μmol/L oxythiamine chloride. The culture conditions of MIA PaCa-2 cells are specified in the Materials and Methods, under the heading; Cell Culture and Isotopic Labeling. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2835986/pdf/nihms-180079.pdf |
|
Source | Literature - own formulation |
Chemically defined > Unspecified | Unspecified |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |