Cell line/type | Osteosarcoma cells |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM/F12 medium Zhou et al. 2010 |
Zhou, S., Li, F., Xiao, J., Xiong, W., Fang, Z., Chen, W., & Niu, P. (2010). Isolation and identification of cancer stem cells from human osteosarcom by serum-free three-dimensional culture combined with anticancer drugs. Journal of Huazhong University of Science and Technology [Medical Sciences], 30(1), 81-84. In this study, cancer stem cells (CSCs) from human osteosarcoma by serum-free three-dimensional culture combined with anticancer drugs were isolated and identified. The primary cells derived from human osteosarcoma were digested by trypsin to prepare a single-cell suspension, and mixed homogeneously into 1.2% alginate gel. Single-cell alginate gel was cultured with serum-free DMEM/F12 medium. Epirubicin (0.8 microg/mL) was added to the medium to enrich CSCs. It is believed that cells obtained from osteosarcoma by serum-free three-dimensional culture combined with anticancer drugs are cancer stem cells. The formulation of the serum-free DMEM/F12 based medium is listed in the Materials and Methods, under the heading; 1.1 Serum-free Culture of Osteosarcoma Cells. https://link.springer.com/content/pdf/10.1007%2Fs11596-010-0114-4.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |