Cell line/type | Huh7 (hepatocarcinoma cell line) |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM supplemented Abe et al. 2007 |
Abe, K. I., Ikeda, M., Ariumi, Y., Dansako, H., & Kato, N. (2007). Serum-free cell culture system supplemented with lipid-rich albumin for hepatitis C virus (strain O of genotype 1b) replication. Virus research, 125(2), 162-168. The aim of this study was to develop a system for robust HCV RNA replication in a serum-free cell culture. Although HuH-7 cells grew in serum-free medium in the presence of selenium, HuH-7 cells under these conditions did not support HCV RNA replication in long-term culture. Among the supplements tested, serum-free medium with lipid-rich albumin (LRA) was found to yield robust HCV RNA replication. HCV proteins were detected for more than 9 months in serum-free medium supplemented with LRA. This is the first report to demonstrate a long-term, serum-free cell culture that successfully maintained robust HCV RNA replication. The serum-free, DMEM based medium is supplemented with sodium-selenite and LRA as specified in the Materials and Methods, under the heading 2.2. Cell cultures. https://www.sciencedirect.com/science/article/pii/S0168170206003947 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |