Cell line/type | Huh7 (hepatocarcinoma cell line) |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM/F12-ITS Akazawa et al. 2011 |
Akazawa, D., Morikawa, K., Omi, N., Takahashi, H., Nakamura, N., Mochizuki, H., ... & Wakita, T. (2011). Production and characterization of HCV particles from serum-free culture. Vaccine, 29(29-30), 4821-4828. In this study, the authors aimed to establish highly efficient HCV particle purification procedures using the following serum-free culture of HCV particles. First, naive human hepatoma Huh7 cells were grown in serum-free medium that was supplemented with human-derived insulin, transferrin and sodium selenite. Then, in vitro transcribed JFH-1 or J6/JFH-1 chimeric HCV-RNA was transfected into the serum-free conditioned Huh7 cells. Infectious HCV was secreted into the culture supernatant with the same efficiency as that from cells cultured in FBS-containing medium. For serum-free culture, the cells were conditioned and cultured in DMEM/F-12 supplemented with Insulin-Transferrin- Selenium-X (ITS). https://ac.els-cdn.com/S0264410X11006141/1-s2.0-S0264410X11006141-main.pdf?_tid=a31cca32-53e3-410d-a8e9-1bb979a6e21c&acdnat=1533804578_27998e14bf5ac51bb67a94315e759fd5 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Unspecified | Unspecified |