Cell line/type | HCEC-12 (human corneal endothelial cell line) |
---|---|
Species | Human |
Animal free | Unspecified |
Product | Endothelial serum-free medium |
Jäckel, T., Knels, L., Valtink, M., Funk, R. H., & Engelmann, K. (2011). Serum-free corneal organ culture medium (SFM) but not conventional minimal essential organ culture medium (MEM) protects human corneal endothelial cells from apoptotic and necrotic cell death. British Journal of Ophthalmology, 95(1), 123-130. The aim of this study was to evaluate the influence of organ culture media on corneal endothelial cell survival. he human corneal endothelial cell line HCEC-12 was cultured in five different media: human corneal endothelial cell (HCEC) growth medium (F99(HCEC)), standard minimal essential corneal organ culture medium (MEM)+2% fetal calf serum (FCS), MEM+5% FCS, and humanised, endothelial serum-free medium (SFM) (with and without antibiotics). The number of apoptotic cells in untreated control cultures was significantly higher in MEM compared with F99(HCEC) and SFM. Staurosporine treatment induced apoptosis in all tested cultures to varying degrees. Cells cultured in MEM showed stronger staining for cleaved caspase-3, cleaved caspase-8, Bax, Bcl-2 and cleaved PARP, increased sub-G1 DNA content, more propidium iodide- and YO-PRO-1-positive cells, and more mitochondria with depolarised membranes. All parameters were significantly higher in MEM compared with F99(HCEC) and SFM. SFM cultures were significantly less susceptible to cell stress. In conclusion, SFM is superior to MEM in promoting HCEC survival. Endothelial serum-free medium is a commercial product. The precise formulation is proprietary. https://bjo.bmj.com/content/bjophthalmol/95/1/123.full.pdf |
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Source | Literature - modified commercial product |
Chemically defined > Unspecified | Unspecified |
Contains phenol red > Yes | Yes |
Antibiotics free > Unspecified | Optional |