Cell line/type | breast CSCs (cancer stem cells) |
---|---|
Species | Human |
Animal free | No |
Product | K-SFM |
Liu, Z. Z., Chen, P., Lu, Z. D., Cui, S. D., & Dong, Z. M. (2011). Enrichment of breast cancer stem cells using a keratinocyte serum-free medium. Chinese medical journal, 124(18), 2934-2936. In this study, K-SFM was used to enrich cancer stem cells (CSCs) from two breast cancer cell lines and a primary culture of breast cancer. RPMI-1640 supplemented with 10% FCS was used as a control. Much higher percentage of CSCs was achieved with K-SFM: 17.3% for MCF-7 cells, 17.4% for SKBR-3, and 20.0% for primary breast cancer culture. Less than 1% CSC was achieved using RPMI-1640 supplemented with 10% FCS. In comparison to the CSCs obtained with RPMI-1640, CSCs in the K-SFM expressed higher levels of Oct-4, ABCG2, Nanog and N-cadherin, and lower level of E-cadherin. K-SFM is an optimal culture medium to maintain and to enrich breast CSCs. Keratinocyte serum-free medium (K-SFM) is a defined medium used to support the growth of primary keratinocytes and embryonic stem cell. K-SFM is a commercially available medium. The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pubmed/22040505 |
|
Source | Literature - commercial |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |