Cell line/type | Retinal cancer stem-like cells |
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Species | Human |
Animal free | Yes |
Product | DMEM/F12 Ma et al. 2011 |
Ma, B., Lei, X., GuAN, Y., MOu, L. S., YuAN, Y. F., Yue, H., ... & Qian, J. (2011). Maintenance of retinal cancer stem cell-like properties through long-term serum-free culture from human retinoblastoma. Oncology reports, 26(1), 135-143. In this study, a long-term culture was established from human retinoblastoma that have cancer stem cell-like properties. Fresh tumor tissue was digested and cultured in serum-free medium. Tumor cells expanded as floating spheres for more than 30 passages. Sphere-forming cells overexpressed stem cell genes Oct4, Nestin and Pax6. Immunostaining of spheres showed positivity for Nestin, Pax6 and also ABCG2. In contrast, differentiated cells derived from these spheres expressed high levels of mature retinal cell markers MAP2, GFAP, recoverin, Opsin B and Nrl, and showed immunoreactivity for NF200, GFAP, recoverin and PKCalpha. Furthermore, both CD44 and CD133 were highly expressed in sphere-forming cells vs. differentiated cells. Sphere-forming cells displayed higher chemoresistance to carboplatin as opposed to differentiated cells. Moreover, intraocular injection of as few as 2x103 sphere-forming cells into NOD/SCID mice gave rise to new tumors similar to the original patient tumors. These results revealed that the sphere-forming cells preserved their stem cell properties and tumorigenicity, even after long-term culture. The DMEM/F12 based, serum-free medium is supplemented with different components as specified in the Materials and Methods. https://www.spandidos-publications.com/or/26/1/135/download |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > Yes | Yes |
Contains phenol red > Yes | Yes |