Cell line/type | ESC-HCs (ESC-derived hematopoietic cells) |
---|---|
Species | Mouse |
Animal free | No |
Product | StemPro34 medium Lesinski et al. 2012 |
Lesinski, D. A., Heinz, N., Pilat-Carotta, S., Rudolph, C., Jacobs, R., Schlegelberger, B., ... & Schiedlmeier, B. (2012). Serum‐and stromal cell‐free hypoxic generation of embryonic stem cell‐derived hematopoietic cells in vitro, capable of multilineage repopulation of immunocompetent mice. Stem cells translational medicine, 1(8), 581-591. In this study, the authors showed that long-term reconstituting HSCs can be efficiently generated in the absence of serum and without stromal cell support under hypoxic conditions by the stepwise addition of cytokines to promote hematopoietic specification and expansion of differentiating mouse ESCs. Cells were cultured in serum-free StemPro34 medium supplemented with different components as specified in the Materials and Methods, under the headings; Embryonic Stem Cell Culture, Retroviral Transduction, |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |