Cell line/type | iPS/ES-derived hepatocyts |
---|---|
Species | Human |
Animal free | No |
Product | DMEM/F12-KSR Nakamura et al. 2012 |
Nakamura, N., Saeki, K., Mitsumoto, M., Matsuyama, S., Nishio, M., Saeki, K., ... & Toyoda, M. (2012). Feeder-free and serum-free production of hepatocytes, cholangiocytes, and their proliferating progenitors from human pluripotent stem cells: application to liver-specific functional and cytotoxic assays. Cellular Reprogramming (Formerly" Cloning and Stem Cells"), 14(2), 171-185. In this study, the authors have established a serum- and feeder-free culture system for the efficient differentiation of multifunctional hepatocytes from human embryonic stem (ES) cells and three entirely different induced pluripotent stem (iPS) cells (including vector/transgene-free iPS cells generated using Sendai virus vector) without cell sorting and gene manipulation. This differentiation culture system provides mature functional hepatocytes, cholangiocytes, and their progenitors with proliferative potential from a wide variety of human pluripotent stem cells. The formulation of the DMEM/F12-KSR based serum-free media is listed in the Materials and Methods, under the heading; Hepatocyte differentiation of human iPS and ES cells in nonfeeder and serum-free culture. https://www.liebertpub.com/doi/pdf/10.1089/cell.2011.0064 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |