UltraCULTURE^® Serum-free medium, without L-Glutamine, 500 mL (BE12-725F)

UltraCULTURE^® is a complete, all purpose medium designed for the cultivation of a wide variety of adherent and non-adherent mammalian cell types. The medium is supplemented with recombinant human insulin, bovine transferrin, and a purified mixture of bovine serum proteins. The total protein concentration of UltraCULTURE^® Medium is approximately 3 mg/ml.

UltraCULTURE^® Medium does not contain L-glutamine; please add 5 ml of 200 mM L-glutamine solution (Cat. No. 17-605 or 17-905) prior to use.

UltraCULTURE^® Medium can be supplemented with Cryoprotective Medium (Cat. No. 12-769E) to cryopreserve cells in a serum-free environment.

The formulation for UltraCULTURE^® Medium has been submitted to the FDA as a Master File. Permission to cross reference the Master File may be obtained by contacting the Regulatory Affairs Department.

Turbidity may develop in UltraCULTURE^® Medium, quality tests have determind that turbidity will not alter the performance of the product.

Moving to serum free cell culturing might require weaning, contact Lonza (https://bioscience.lonza.com/contact/lonza) to get protocols for practice.

Benefits

• General purpose medium to replace classical media in combination with serum

Applications

• Cultivation of adherent and non-adherent mammalian cells
• Generation of viral particles for vaccine production
• Clinical immunotherapy applications
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