Cell line/type | Ovarian tumor cells |
---|---|
Species | Human |
Animal free | No |
Product | DMEM/F12 Pan et al. 2012 |
Pan, Z., Hooley, J., Smith, D. H., Young, P., Roberts, P. E., & Mather, J. P. (2012). Establishment of human ovarian serous carcinomas cell lines in serum free media. Methods, 56(3), 432-439. In this article, a method is described using a defined medium, which allows for the successful establishment of continuous cell cultures from a minority cell type within serous ovarian cancers. The cell lines established using these methods grow in serum-free hormone-supplemented medium either as a monolayer on a matrix or as tumor spheres in suspension. The formulation of the DMEM/F12 based, serum-free medium is listed in the Cell culture materials, under the heading; 2.2.2. Growth medium and in Table 1 Cell culture medium and supplements. https://ac.els-cdn.com/S1046202312000503/1-s2.0-S1046202312000503-main.pdf?_tid=9636f9e5-ff3a-498a-a77e-f79a7572e5c6&acdnat=1533545801_754a3856f272b11ab4ef80348f7547df |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |