Cell line/type | MDM (monocyte-derived macrophages) |
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Species | Human |
Animal free | Yes |
Product | XVivo 10 Rey-Giraud et al. 2012 |
Rey-Giraud, F., Hafner, M., & Ries, C. H. (2012). In vitro generation of monocyte-derived macrophages under serum-free conditions improves their tumor promoting functions. PloS one, 7(8), e42656. In this study, differences in cell morphology were observed as well as increased surface receptor expression levels in serum-containing culture whereas similar or higher cytokine production levels were detected under serum-free culture conditions. More importantly, monocyte-derived macrophages (MDM) differentiated under serum-free conditions displayed enhanced tumoricidal activity for M1 and tumor promoting property for M2 macrophages in contrast to MDM differentiated in the presence of serum. Moreover, evaluation of MDM phagocytic activity in serum-free condition resulted in greater phagocytic properties of M2 compared to M1. The serum-free medium, XVivo 10, media specifically developed for hematopoietic cells, was supplemented with cytokines as specified in the Materials and Methods, under the heading; In vitro generation of monocyte-derived macrophages (MDM). XVivo 10 is a commercial product. The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412794/pdf/pone.0042656.pdf |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |