Cell line/type | Primary glioma (spheroids) |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM-F12 Balvers et al. 2013 |
Balvers, R. K., Kleijn, A., Kloezeman, J. J., French, P. J., Kremer, A., van den Bent, M. J., ... & Lamfers, M. L. (2013). Serum-free culture success of glial tumors is related to specific molecular profiles and expression of extracellular matrix–associated gene modules. Neuro-oncology, 15(12), 1684-1695. In this study, a cohort of 261 glioma samples from varying histological grades was documented for serum-free (SF) culture success and clinical outcome. Gene expression and single nucleotide polymorphism arrays were interrogated on a panel of tumors for comparative analysis of SF+ (successful cultures) and SF- (unsuccessful cultures). All SF+ cultures are derived from tumors that are isocitrate dehydrogenase 1 (IDH1) wild type, chromosome 7 amplified, and chromosome 10q deleted. SF- cultures derived from IDH1 mutant tumors demonstrated a fade-out of mutated cells during the first passages. SF+ tumors were enriched for The Cancer Genome Atlas Classical subtype and intrinsic glioma subtype-18. Comparative gene ontology analysis between SF+ and SF- tumors demonstrated enrichment for modules associated with extracellular matrix composition, Hox-gene signaling, and inflammation. SF cultures are derived from a subset of parental tumors with a shared molecular background including enrichment for extracellular matrix-associated gene modules. The formulation of the DMEM-F12 based serum-free medium is listed in the Materials and Methods, under the heading, Glial Stem-like Cell Cultures and Serum-supplemented Cultures From Glioma Resection Specimens. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829587/pdf/not116.pdf |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |