Cell line/type | BMC (Bone marrow cell) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Species | Human | ||||||||||||||||||||
Animal free | No | ||||||||||||||||||||
Product | X-VIVO tm 10 Serum-free hematopoietic cell medium, without gentamicin and PR with recombinant transferrin | ||||||||||||||||||||
X-VIVOTM 10, Serum-free hematopoietic cell medium, with recombinant transferrin and L-Glutamine, without gentamicin and phenol red, xenofree, 1 L (BE02-055Q) The X-VIVOTM 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 106 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peripheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 106 cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2. X-VIVOTM 10 Media is available as a 1X liquid in two convenient formulations. There are 3 different formulations of X-VIVOTM 10:
Benefits
Applications
|
|||||||||||||||||||||
Source | Lonza | ||||||||||||||||||||
Contains phenol red | No |