Cell line/type | BMC (Bone marrow cell) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Species | Human | ||||||||||||||||||||
Animal free | No | ||||||||||||||||||||
Product | X-VIVO tm 10, Serum-free hematopoietic cell medium without gentamicin and PR | ||||||||||||||||||||
X-VIVOTM 10, Serum-free hematopoietic cell medium, with L-Glutamine, without gentamicin and phenol red, xenofree, 1 L (EU: BE04-743Q/ US: 04-743Q) The X-VIVOTM 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 106 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peripheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 106 cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2. X-VIVOTM 10 Media is available as a 1X liquid in two convenient formulations. There are several X-VIVOTM 10 Media available, here an overview:
Benefits
Applications
https://bioscience.lonza.com/lonza_bs/BE/en/Culture-Media-and-Reagents/p/000000000000183885/X-VIVO-10-Serum-free-Hematopoietic-Cell-Medium |
|||||||||||||||||||||
Source | Lonza | ||||||||||||||||||||
Contains phenol red | No |