Cell line/type | SVF (stromal vascular fraction) cells |
---|---|
Species | Human |
Animal free | Yes |
Product | human endothelial-SFM |
Touroo, J. S., Dale, J. R., & Williams, S. K. (2013). Bioengineering human blood vessel mimics for medical device testing using serum-free conditions and scaffold variations. Tissue Engineering Part C: Methods, 19(4), 307-315. The purpose of this study was to eliminate animal serum and modify scaffold properties in human blood vessel mimic (BVM) preparation. The outcomes of this study support the hypothesis that BVMs developed using serum-free conditions are affected by scaffold variations and exhibit tissue growth over implanted medical devices. Ultimately, employing serum-free methods could lead to controlled, reproducible BVM production and interpretable, human-specific results in studies of device-tissue interaction, toxicity, and other vascular phenomena; however, comparisons to in vivo biological responses and incorporation of defined blood vessel cells will be critical to validating the serum-free BVM as an appropriate device testing alternative. The serum-free medium selected for these experiments, human endothelial-SFM, has been utilized in culturing human umbilical vein, human umbilical arterial, and human microvascular endothelial cells, Battista et al. 1995. Battista, P.J., Bowen, H.J., and Gorfien, S.F. A serum-free medium for the culture of human umbilical vein endothelial cells. Focus 17, 10, 1995. |
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Source | Literature - modified commercial product |
Chemically defined > No | No |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |