Cell line/type | Corneal endothelium cells |
---|---|
Species | Bovine (cow) |
Animal free | No |
Product | DMEM/F12 + platelet releasates |
Chou, M. L., Burnouf, T., & Wang, T. J. (2014). Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate. PloS one, 9(6), e99145. In this study, the growth and viability of corneal endothelium cells are compared in DMEM-F12 medium supplemented with different combinations of components, including 2.5% approximately 10% of the platelet releasates. Corneal endothelium cells expanded in platelet releasates exhibited good adhesion and a typical hexagonal morphology. Their growth and viability were enhanced when using the complement-inactivated platelet releasate at a concentration of 10%. Immunostaining and Western blots showed that CECs maintained the expressions of four important membrane markers: Na-K ATPase alpha1, zona occludens-1, phospho-connexin 43 and N-cadherin. In conclusion, this study provides the first proof-of-concept that human platelet releasates can be used for ex vivo expansion of corneal endothelium cells. The formulation of DMEM-F12 medium supplemented with platelet releasates is specified in the Materials and Methods, under the heading; CEC Isolation and Culture Conditions . https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063748/pdf/pone.0099145.pdf |
|
Source | Literature - own formulation |
Chemically defined > No | No |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |