Cell line/type | DCs (dendritic cells) |
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Species | Murine (Mouse or rat) |
Animal free | Unspecified |
Product | X-VIVO 15/20 Kim et al. 2007 |
Kim, S. J., & Diamond, B. (2007). Generation and maturation of bone marrow-derived DCs under serum-free conditions. Journal of immunological methods, 323(2), 101-108. In this study, the authors compared several serum-free culture conditions with respect to survival, differentiation, activation, and cytokine profile of murine dendritic cells (DCs) progenitors. DC progenitors can survive only in some serum-free conditions. DCs grown in serum-free medium display a higher expression of activation markers upon stimulation. They produce increased IL-12 and decreased IL-6 following stimulation. Furthermore, DCs derived under serum-free conditions may express unusual surface markers, B220 and Ly6C/G, implying an increased differentiation to plasmacytoid DCs (pDCs). RPMI 1640 supplemented with 100 U/ml penicillin and streptomycin, 2 mM glutamate, and 10% heat-inactivated fetal bovine serum (FBS) and Ultralow IgG FBS were used as serum-containing culture conditions. For serum-free culture of DCs, CellGro GMP serum-free media , X-VIVO 15, XVIVO20 were used. Murine bone marrow-derived DCs grew only in X-VIVO serum-free media with a slightly lower yield than grown in serum-containing medium. Although serum-free culture resulted in low number of cells, the purity of DCs, based on CD11cpositive cells, was equivalent. CD11c+ cells from serum-free culture displayed phenotypic maturity in the absence of IL-4. Functional activation was measured by secretion of IL-12. Serum-free cultured DCs secreted 2–4 times more IL-12 measured by ELISA. The same DCs displayed severely decreased secretion of another pro-inflammatory cytokine, IL-6. In conclusion, two serum-free media from Bio-Whittaker, X-VIVO15 and X-VIVO20, led to a reasonable number of DCs, compared to the serum-containing culture condition. X-VIVO 15 and X-VIVO-20 are commercial products, their formulation is proprietary. https://ac.els-cdn.com/S0022175907000920/1-s2.0-S0022175907000920-main.pdf?_tid=5e75323e-db15-4acb-aed0-d65ba3a693b2&acdnat=1534430137_4594fd0bea9b9c1b80daf26474d1ed07 |
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Source | Literature - commercial |
Chemically defined > Yes | Yes |
Contains phenol red > Unspecified | Unspecified |
Antibiotics free > Unspecified | Unspecified |