Cell line/type | HepaRG, HPR116 (human hepatoma) |
---|---|
Species | Human |
Animal free | No |
Product | Williams E medium Klein et al. 2014 |
Klein, S., Mueller, D., Schevchenko, V., & Noor, F. (2014). Long‐term maintenance of HepaRG cells in serum‐free conditions and application in a repeated dose study. Journal of Applied Toxicology, 34(10), 1078-1086. In this study, was shown that HepaRG cells can be maintained in optimized serum-free conditions for 30 days without viability change and with high CYP activity. In this study, we show that the HepaRG cells can be maintained The long-term viability and CYP activities in SFM conditions were superior to those reported for PHH. For the two serum-free conditions, SFM1 and SFM2, a slight increase in the percentage of viable cells was observed after day 18 of cultivation. The cells were maintained in Williams Medium E supplemented with different components as specified by the Materials and Methods, under the heading; Cell Culture. The serum-free media compositions are summarized in Table 1. https://www.spandidos-publications.com/or/32/2/845/download |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |