Mathiesen, C. K., Jensen, T. B., Prentoe, J., Krarup, H., Nicosia, A., Law, M., ... & Gottwein, J. M. (2014). Production and characterization of high-titer serum-free cell culture grown hepatitis C virus particles of genotype 1–6. Virology, 458, 190-208.
DOI: 10.1016/j.virol.2014.03.021.

In this study, the generation of genotype 1-6 serum-free HCVcc (sf-HCVcc) from Huh7.5 hepatoma cells cultured in adenovirus expression medium is described.

Adenovirus Expression Medium (AEM) is an animal-origin free cell culture medium without components directly derived from human or animal sources. AEM is a commercially available, proprietary product.