Cell line/type | WiDr, SW480 and Colo205 (colorectal cancer cells) |
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Species | Human |
Animal free | Unspecified |
Product | OncoMiD |
Mélin, C., Perraud, A., du Puch, C. B. M., Loum, E., Giraud, S., Cardot, P., ... & Mathonnet, M. (2014). Sedimentation field flow fractionation monitoring of in vitro enrichment in cancer stem cells by specific serum-free culture medium. Journal of Chromatography B, 963, 40-46. In this study, the in vitro use of OncoMiD for colon, a CRC-specific primary cell culture medium, to enrich for colorectal cancer (CRC) cell lines in cancer stem cells (CSC) is described. This work strongly suggests that cells from CRC cell lines cultured in OncoMiD for Colon medium were initially able to proliferate. Then, an intermediate state with a balance between proliferation/apoptosis was reached, with the ability to form non-adherent clusters that strongly expressed CD44and EpCAM. These characteristics are common properties of CSC. Consequently, OncoMiD for Colon medium appears to be a valuable tool to enrich in vitro cell cultures in undifferentiated cells comprising both activated (responsible for tumor growth) and quiescent (responsible for resistance) CSC. This enrichment could be due to cellular de-differentiation of a portion of cells from the CRC lines. OncoMiD is a commercial product. The precise formulation is proprietary. https://www.sciencedirect.com/science/article/pii/S157002321400347X |
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Source | Literature - commercial |
Chemically defined > Yes | Yes |