Cell line/type | NT2/D1 |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM/F12-ITS Jasnic-Savovic et al. 2015 |
Jasnic-Savovic, J., Klajn, A., Milivojevic, M., Mojsin, M., & Nikcevic, G. (2015). Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation. Alternatives to laboratory animals: ATLA, 43(1), 9-18. In this study, RA-induced differentiation of NT2/D1 cells were compared in serum-containing and serum-free media, by using SOX3 protein levels as a marker of differentiation. It was found that NT2/D1 cells can be successfully grown under serum-free conditions, and that the presence or absence of serum does not affect the level of SOX3 protein after a 48-hour RA induction. However, six days of RA treatment resulted in a marked increase in SOX3 protein levels in serum-free media compared to serum-containing media, indicating that serum might have an inhibitory effect on the expression of this neural differentiation marker. The cells were cultured in DMEM/F12 supplemented with ITS as specified in the Materials and Methods, under the heading; Cell culture. https://www.ncbi.nlm.nih.gov/pubmed/25802994 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |