Cell line/type | PMSCs (Placenta-derived mesenchymal stem cells) |
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Species | Human |
Animal free | Yes |
Product | Guangzhou Jinan Biomedicine Research and Development |
The formulation of this serum-free medium was published in forms of patent and can be accessible via Google browser. It was mentioned that the optimum formula of the serum free medium provided by the invention is: DMEM basic medium which can be of high glycoform or low-sugar type, vitamin H 5ug/ml, GSH (reduced glutathione) 10ug/ml, recombinant human insulin 6.5mg/ml, human serum albumin 1mg/ml, Transferrin-iron complexes 6ug/ml, Fibroblast growth factor (FGF) 10 ng/ml, Urogastrone 5ng/ml, human stem cell factor (SCF) 5ng/ml and Honokiol (a polyphenol isolated from Magnolia officinalis) 25uM. It seems that Honokiol serves as natural antimicrobial agents besides antioxidants (corroborated by patent citation titled "Antimicrobial Activity of Honokiol and Magnolol Isolated from Magnolia officinalis" and other related literature) in replacement of synthetic antibiotics commonly added in culture medium formulation. Note: Urogastrone is alias name for epidermal growth factor or EGF (https://www.sciencedirect.com/topics/immunology-and-microbiology/urogastrone). Vitamin H is another name for Biotin (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034481/) https://patents.google.com/patent/CN105112362A/en |
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Source | Internet |
Chemically defined > Yes | Yes |
Guangzhou Jinan Biomedicine Research and Development - Serum-free medium for culturing placenta mesenchymal stem cells https://patents.google.com/patent/CN105112362A/en |