Cell line/type | WJ-MSC (Wharton’s Jelly Mesenchymal stem cells) |
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Species | Human |
Animal free | Yes |
Product | Mesencult XF Swamynathan et al. 2014 |
Swamynathan, P., Venugopal, P., Kannan, S., Thej, C., Kolkundar, U., Bhagwat, S., ... & Balasubramanian, S. (2014). Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study. Stem cell research & therapy, 5(4), 88. In this study, a complete xeno-free, serum-free culture condition for isolation, expansion and characterization of WJ-MSCs was established, to eliminate the use of animal components right from initiation of explant culture to clinical scale expansion and cryopreservation. Growth kinetics, in vitro differentiation capacities, immunosuppressive potential and immunophenotypic characterization of the cells expanded in serum-free media have been compared against those cultured under standard fetal bovine serum (FBS) containing medium. Colony-forming frequency and genomic stability of the large scale expanded cells were compared. Secretome analysis was performed to compare the angiogenic cytokines and functional angiogenic potency was proved by Matrigel assays. Results presented in this report identify one such serum-free, xeno-free medium for WJ expansion. Cells cultured in serum-free, xeno-free medium exhibit superior growth kinetics and functional angiogenesis, alongside other MSC characteristics. t WJ-MSCs cultured and expanded in Mesencult XF, SF Medium retain all necessary characteristics attributed to MSC for potential therapeutic use. Mesencult XF is a commercially available product. The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4247668/pdf/13287_2013_Article_401.pdf |
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Source | Literature - commercial |
Chemically defined > Yes | Yes |