Cell line/type | WJ-MSC (Wharton’s Jelly Mesenchymal stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | XcytePLUS™ media |
Riordan, N. H., Madrigal, M., Reneau, J., de Cupeiro, K., Jiménez, N., Ruiz, S., ... & Patel, A. N. (2015). Scalable efficient expansion of mesenchymal stem cells in xeno-free media using commercially available reagents. Journal of translational medicine, 13(1), 232. In this study, XcytePLUS media, a human platelet lysate based product, was used to supplement the culture medium at 5, 7.5 and 10% and compared to fetal calf serum at 10%, for human umbilical cord MSC expansion. Properties of the expanded cells were investigated. This study demonstrated equivalent or superior effects of human platelet lysate compared to standard FCS supplemented media, based on doubling rate, without loss of identity or function, as demonstrated with flow cytometry characterization. Differentiation into osteocytes, adipocytes and chondrocytes was comparable from cells expanded in either media supplement. These data support the implementation of human platelet lysate supplemented media as an alternative to xenogeneic containing preparations which may lead to safer MSC products with therapeutic uses. Equivalent or superior proliferation, and differentiation activity of WJ-MSC cultured in XcytePLUS™ media as compared to FCS. The cells were cultured in XcytePLUS media, aMEM supplemented with 2 mM GlutaMax and 10% XcytePLUS™. XcytePLUS™ is a human platelet lysate based, commercial product. The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4504159/pdf/12967_2015_Article_561.pdf |
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Source | Literature - modified commercial product |
Chemically defined > No | No |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |