Cell line/type | Primary brain tumour cells |
---|---|
Species | Human |
Animal free | No |
Product | UltraCULTURE™ Sandén et al. 2015 |
Sandén, E., Eberstål, S., Visse, E., Siesjö, P., & Darabi, A. (2015). A standardized and reproducible protocol for serum-free monolayer culturing of primary paediatric brain tumours to be utilized for therapeutic assays. Scientific reports, 5, 12218. In this study, a simple, non-expensive and reproducible serum-free cell culture protocol is defined for establishment and propagation of primary paediatric brain tumour cultures as adherent monolayers. The success rates for the establishment of primary cultures (including medulloblastomas, atypical rhabdoid tumour, ependymomas and astrocytomas) were 65% (11/17) and 78% (14/18) for sphere cultures and monolayers respectively. Monolayer culturing was particularly feasible for less aggressive tumour subsets, where neurosphere cultures could not be generated. The results suggest that this culture method could increase the availability and comparability of clinically representative in vitro models of paediatric brain tumours, and encourages further molecular evaluation of serum-free monolayer cultures. Cells were cultured in UltraCULTURE™ cell culturing medium supplemented with different components as specified in the Methods, under the heading; Tumour dissociation and cell culturing. UltraCULTURE™ SFM is a commercial product. It consists of a DMEM:F12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The formulation for UltraCULTURE™ Medium has been submitted to the FDA as a Master File. Permission to cross-reference the Master File may be obtained by contacting the Regulatory Affairs Department.The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4505308/pdf/srep12218.pdf |
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Source | Literature - modified commercial product |
Chemically defined > Unspecified | Unspecified |
Antibiotics free > No | No |
Contains phenol red > Unspecified | Unspecified |