Silva, A. C., Simão, D., Küppers, C., Lucas, T., Sousa, M. F., Cruz, P., ... & Alves, P. M. (2015). Human amniocyte‐derived cells are a promising cell host for adenoviral vector production under serum‐free conditions. Biotechnology journal, 10(5), 760-771.

In this study, the generation of a new human amniocyte-derived cell line named 1G3 is described and showed that it can be used as a very promising cell host for AdV production in serum-free conditions, allowing for production in high cell density cultures and avoiding the typical cell density effect observed for HEK293. Using stirred tanks bioreactors, it was possible to grow 1G3 cells to cell densities of up to 9 × 106 cells/mL using serum-free media. Overall, the results clearly demonstrate the potential of the human amniocytederived newly established cell line 1G3 for AdV production in a serum-free scalable process, paving the way for further process improvements based on fed-batch or perfusion strategies.

Cells were adapted to grow in suspension cultures as single cells in serum-free Ex-Cell™ 293 medium supplemented with 4 mM glutamine as specified in the Materials and Methods, under the heading; 2.3 Stirred cultures. Ex-Cell™ 293 medium is a proprietary, commercially available medium.