Cell line/type | CD38+CD138+CD31+ plasma cells |
---|---|
Species | Human |
Animal free | Yes |
Product | BPFM |
Bonnaure, G., Gervais-St-Amour, C., & Néron, S. (2016). Bone marrow mesenchymal stem cells enhance the differentiation of human switched memory B lymphocytes into plasma cells in serum-free medium. Journal of immunology research, 2016. In this study, low oxygen concentrations and coculture with mesenchymal stem cells (MSC) from human bone marrow were tested. All cultures were performed using an animal protein-free medium. This model enables the generation of high proportions of CD38+CD138+CD31+ plasma cells (>/=50%) when CD40-activated switched memory B lymphocytes were cultured in direct contact with mesenchymal stem cells. In these cultures, the secretion of CXCL12 and TGF-beta, usually found in the bone marrow, was linked to the presence of MSC. The level of oxygen appeared less impactful than the contact with MSC. This study shows for the first time that expanded switched memory B lymphocytes can be differentiated into plasma cells using exclusively a serum-free medium. The formulation of animal protein-free medium BPFM is described in Néron et al 2011. |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |