Cell line/type | hESC line H9 |
---|---|
Species | Human |
Animal free | Yes |
Product | BRASTEM |
Marinho, P. A. N., Vareschini, D. T., Gomes, I. C., Paulsen, B. D. S., Furtado, D. R., Castilho, L. D. R., & Rehen, S. K. (2012). Xeno-free production of human embryonic stem cells in stirred microcarrier systems using a novel animal/human-component-free medium. Tissue Engineering Part C: Methods, 19(2), 146-155. In this study, human ESC were cultered in stirred microcarrier (MC) systems using an animal/human-component-free medium. The method developed to culture cells on suspended beads combined the use of polymeric MCs in stirred vessels with an optimized culture medium free of supplements of animal and human origin. Considering the high cell growth achieved and the maintenance of pluripotency observed, our data confirm stirred MC systems as the currently best alternative for scaling-up production of human ESC. The use of the xeno-free, chemically defined, animal/human-component-free medium BRASTEM was reported here for the first time and showed that its use in combination with the xeno-free Hillex II MCs provides a promising regulatory-friendly way to produce large amounts of low-immunogenic human ESC. Brastem culture medium is pattented by the Federal University of Rio de Janeiro and at the University of California at San Diego. https://www.liebertpub.com/doi/pdfplus/10.1089/ten.tec.2012.0141 |
|
Source | Literature - commercial |
Chemically defined > Yes | Yes |