Cell line/type | hiPSCs-derived xf-lt-NES (neuroepithelial-like stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | StemFit®AS200 |
Isoda, M., Kohyama, J., Iwanami, A., Sanosaka, T., Sugai, K., Yamaguchi, R., ... & Okano, H. (2016). Robust production of human neural cells by establishing neuroepithelial-like stem cells from peripheral blood mononuclear cell-derived feeder-free iPSCs under xeno-free conditions. Neuroscience research, 110, 18-28. In this study, a procedure to obtain xeno-free long-term self-renewing neuroepithelial-like stem cells (xf-lt-NES cells) from feeder-free hiPSCs was established using a newly developed xeno-free medium, StemFit((R))AS200. xf-lt-NES cells were cultured for long periods in StemFit((R))AS200 while retaining normal karyotypes, NS/PC marker expression and differentiation capacity for neuronal and glial differentiation in vitro and in vivo. Furthermore, the cells were cryopreserved using a defined serum-free freezing reagent, which demonstrated the feasibility of this xeno-free culture system for large-scale lt-NES cell production and cell banking. Taken together, this system represents a promising approach for the manufacture of clinically relevant products for cell therapy using NS/PCs. The components of StemFit®AS200 are listed in the Materials and Methods, under the heading; 2.1. Components of StemFit®AS200. The precise concentrations of the medium formula are proprietary. https://www.sciencedirect.com/science/article/pii/S0168010216300074?via%3Dihub |
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Source | Literature - commercial |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |